I'm currently working in the Trudeau laboratory at the Université de Montréal in Canada, renowned for its cyclic voltammetry for dopamine detection. After learning about this technique and embarking on an intriguing project, we decided to focus on the voltammetric detection of serotonin. Given my existing installation, I established a voltage curve ranging from +0.2 to +1.0, then to -0.1, and back to +0.2 at frequencies of 300 V/s and 1000 V/s.
After a complete analysis of my detection in solution and in slices at SNr and DRN, there is variability in the detection of the molecule based on the oxidation/reduction principle, in this case serotonin. This variability is observed from one electrode to another, from one sample to another and even between 5-minute stimulation intervals. One potential explanation concerns the detection limit of the electrode, which could be mitigated by the use of Nafion, as reported in several articles, or by the consideration of other as yet unknown factors.
I carried out a thorough review of my approach to processing the raw data, none of which revealed any interpretation errors. In addition, I implemented a protocol with a scan rate of 1000 V/s, as suggested in one paper; however, this did not lead to improved detection. I also examined the difference between reduction and oxidation. However, as the problem persists in the voltammogram, this approach failed to improve detection.
If you have any ideas, questions, or suggestions that come to mind, I'm open to all contributions.
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