I find out some fungi (e.g Beauveria bassiana, Verticillium lecanii, Aschersonia aleyrodis, Paecilomyces fumosoroseus) in the literature that control the whitefly but I can’t find out from where (source) and how (procedure), I can isolate these entomopathogenic fungi, so experts please guide me ..
Hello:
The best entomopathogenic fungi that control whiteflies are Verticillium lecanii and Aschersonia aleyrodis. These fungi attack the nymphal stage. For isolation entomopathogenic fungi the best media is Potato dextrose agar (PDA) and potato dextrose broth, both are common microbiological growth media made from potato infusion, and dextrose, with Chlortetracycline, Chloramphenicol and tartaric acid.
Regards,
Luis Miguel
B. bassiana and M. anisopliae are good candidates, pathogenic to whiteflies. PDA is the best media.
The best fungus for controlling white fly is Lecanicillium lecanii
u can isolate entomopathogenic fungi from soil using selective medium or insect-baiting method was commonly using larvae of Galleria mellonella or Tenebrio molitor as the bait (Meyling, 2007; Vega et al., 2012).
Vega, F. E., N. V. Meyling, J. J Luangsa-Ard and M. Blackwell, 2012. Fungal entomopathogens. In: Insect pathology, Vega, F. E. and H. K. Kaya, 2nd Ed. San Diego: Academic Press, pp: 171-220.
Meyling, N. V. (2007). Methods for isolation of entomopathogenic fungi from the soil environment-laboratory manual.
Hello!
As a lot of people have mentioned above you can isolate them from soil using a selective medium or an insect-baiting method. I recommend you to isolate them from the soil of the crop you are working with because of the different climmate and pH conditions of the soil.
Hello Hafiz,
my PhD thesis was on the efficacy of entomopathogenic fungi against whiteflies. I think the best fungus is Isaria fumosorosea (= Paecilomyces fumosorososeus). Also, Lecanicillium lecanii or L. muscarium can be very good agents to control whiteflies populations. Ashersonia aleyrodis is also working good but is very specific, selective fungus but it occurs in tropical and subtropical areas, so in EU it doesn't occur. The strains of Isaria fumosorosea and L. lecanii are polyphagous so they are able to infect another sucking insects.
You can isolate these fungi from soil or from phylloplane (from natural epizootics). It depends if these fungi occur in the nature around your area. I am from the Czech Republic, so we don't have natural epizootics in the population of sucking insect. Perhaps they are in the Czech Republic, but very very weak, and if yes, it is Lecanicillium.
However, in tropical and subtropical regions, natural epizootics are common (I have seen huge epizootics in the population of aphids and scales in Florida), and strains of EPF are well isolated from infected insect populations.
1) They can be isolated directly on a PDA (we use this) or another nutrient medium. In the case of larger contaminations, it is good to use a selective nutrient medium based on dodine (fungicide active ingredient dodine, PDA, chlorapmhenicol, cycloheximide) for isolation of strains from epizootics.
2) For isolation of EPF from soil, the selective medium based on dodine is using. Most isolated species from soil are Metarhizium spp. (M. brunneum, M. anisopliae, ...), Beauveria spp. (B. bassiana, B. brongniartii, B. pseudobassiana) and Isaria spp. (I. carnea, I. javanica).
Beauveria bassiana and Metarhizium brunneum are also working good against whiteflies but Isaria fumosorosea seems to be much much better. Isaria fumosorosea is kind of fungus can be spread by adults of whiteflies or another insect in the populations (horizontal transmission).
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