Which are the cell lines used to evaluate cytotoxicity of an extract from herbal drug?

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Alok Nahata Popular answer

Lung (A549), prostate (PC-3 and DU-145), neuroblastoma (IMR-32), breast cancer (MCF-7), ovary (IGR-OV-1), acute lymphoblastic leukemia (HL-60), leukemia (THP-1), liver (HEP-2), colon (Colo-205, HCT-15, Caco-2), and cervix (Hela) are some of the cell lines used to study the cytotoxicity of herbal drugs using in vitro cytotoxicity assays like SRB and MTT.

Alok Nahata

Bhavesh Tiwari

Thank you very much Sir.

Shamala Nadaraju

Dr Alok,

I am curious about caco-2 cell lines, this cell line is used to evaluate intestinal permeability for oral drugs , can we also use them for MTT assay?

Sandipan Datta

It will depend on whether you are evaluating the cytotoxicity along with a bioactivity. If you are showing a particular bioactivity for the extracts then you should evaluate cytotoxicity in one of the related cell line. For an example if you are showing hepatoprotective activity then you should choose a liver cell line for cytotoxicity evaluation. Also if you want to show a systemic toxicilty you might pick a panel of cell lines from various end organs like liver brain kidney heart etc.

@Shamala Nadaraju. Yes you can use Caco2 cell lines for MTT assay. Pls refer the following link for referencing:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2902307/

Other than these, many other literature references are available which are relevant with your query. Just google them out. You will find answers in detail (some pdf's are also available) regarding your query.

Thank you Dr Alok.

Dear all,

If i am working on RAW cell lines of macrophages then Is it necessary to carry put cytotoxicity on the same cell lines? Or some other cell lines like Vero?

Doing cytotoxicity in RAW would be more relevant and scientifically correct

Vani C.

You can work with Vero cell lines

Hind Hussein

Dear Sir, you can test toxicity in all cancer cell lines, also on transformed cell lines, because they are different in origin, also found normal cell lines.