Which are better cell lines to study the atherosclerosis model in vitro?

More Deepmala Joshi's questions See All

Can I use soybean, sunflower, or olive oil to prepare a standard curve for lipid estimation in plants or pollen?

I am trying to analyze lipids in plants; specifically pollen by using Sulfo-Phospho-Vanillin (SPV) Method! Can I prepare a standard curve for this estimation using soybean, sunflower, or olive...

01 July 2024 3,267 1 View

To what extent Google Scholars Citations system is reliable?

1. I was shocked to discover yesterday that my research citations on Google Scholar had dropped to 2383 from 2419 a week ago, and they are now up to 2391. 2. My citations three months ago were...

28 June 2024 2,846 2 View

Can Anyone Recommend Papers on OER Mechanism in Perovskites Using DFT?

Hello everyone, I am currently conducting research on the Oxygen Evolution Reaction (OER) mechanisms in perovskite materials and am particularly interested in studies that utilize Density...

03 June 2024 6,295 0 View

What is the rationale for both C- Myc tag and 6xHis tag in same plasmid. Can i use only his tag for mammalian expression?

I wanted to express protein in mammalian cells for purification I need 6XHis tag, however, plasmid synthesis company have vector with Myc and his tag. So do I need both or only his tag.

12 May 2024 941 4 View

Methodology for quantifying particulate matter on leaf surface & Pollen by Scanning Electron Microscopy with energy dispersive X-ray spectroscopy?

I needed a detailed methodology for SEM-EDS analysis for Leaf and pollen!

05 May 2024 4,892 5 View

What are the currently available options for mass marking ants during an experiment?

Hi all, I'm looking to mass mark Solenopsis xyloni ants during an experiment. For this experiment, I'm allowing the whole colony to feed on three feeders, and I want to mass mark all the ants...

13 April 2024 9,988 2 View

How do we use the EMBU scale for assessing parenting style?

Hey, I am doing an research on how parenting styles create negative attachment styles in young adults. I can't seem to understand what's the criteria for assessing and how to use it. It would be...

02 April 2024 7,017 2 View

What is "bonitur scale" and how to implement it in the PGP studies? Is it a good system in current research arena?

Please share the details of the bonitur scale, its significance, and its relevance in current times.

01 April 2024 2,523 0 View

How do I increase my citation count?

Hey all! I am looking for help to increase the citation count for my memristor test research. Memristor test is a relatively new area with limited commercial availability for these nanoscale...

27 March 2024 2,373 2 View

Sections with CMC than OCT for spatial transcriptomics slide using Visium HD?

Has anyone run slide in Visium of tissue sections (Heart, kidney, brain) with CMC than OCT for spatial transcriptomics ???

14 March 2024 243 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Deepmala Joshi

Thank you Dr. Ping

Elisa A Liehn

Smooth muscle cells (generally primary isolated cells, but there are also cell lines). I have worked with rat media and intima smooth muscle cells, endothelial cells, monocyte/macrophages typ M1, and more, depending on the interest!

There are now provider offering "atherosclerosis on the chip"!