I would like to study a role of lactose and its receptor in epilepsy using mouse models, has anyone some suggestion which approach would be better? like use antagonists for this receptor and induce epilepsy or?
Hi I would like to develop some medical assays for example for cancer prevention, or analysis of some markers for some disease which would be good for application in medicine in Georgia country....
04 May 2019 5,102 3 View
I did synthesis of 2 peptides (1-20 amino acids) using MDHA Rink resin, I used HBTU (3.7g in 20ml (DMF)), HOBT (1.35g (29ml) NMP) to get 0.5 M. For each reaction I used 1 mL HBTU solution, 1ml...
10 November 2018 7,622 4 View
Hi all I am interesting if you want to produce some protein and have plasmid, the way is to grow it in bacteria and than isolate with antibody which will bind tag (example FLAG tag etc.) but what...
09 October 2018 8,696 5 View
How to view an article short term if journal is not open access? Is there any website which for example shows content but is not possible to copy?
07 August 2018 10,206 3 View
I am interesting if somebody can suggest in Excel 2016, I have columns and want to write mine values instead this it chooses only automatic range for example from 1 to 40. I want type for example...
07 August 2018 7,341 0 View
I want to study if bacterias are associated with root, can anyone suggest for fluorescent marker which would be better to use? or which method is good (example some staining solution like acridine...
11 December 2017 2,391 5 View
Hi All There is opening laboratory of molecular biology in Georgia country. I am interesting which kind of product can produce (budget 10 000 dollars) molecular biology laboratory to start...
09 October 2017 9,743 0 View
I have some mix of bacteria and would like to know which species there are exactly. Should I send them for sequencing their DNA? or what would be cheap method?
06 July 2017 9,692 10 View
Does anyone know any website which lets at least just to read article of journals which have not open access? if its not possible to download a PDF.
09 October 2016 4,346 4 View
I am interesting can anyone suggest how to track protein transport into the cells not using GFP or any big markers? I would like to minimize the effect of marker (keep size and function of protein...
08 September 2016 6,918 7 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View