Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

Face meshing fails around dimple, how do i split the airfoil?

I am trying to do a flow simulation around this airfoil but everytime the face mesher fails around the dimple, how do i split the airfoil to get a finer mesh?

19 August 2020 4,307 7 View

Can we register patent of research idea before the project is formally accepted/completed? Can we get Intellectual property right of research ideas?

Reputed institutions and academics decline many applications for research degrees and scholarship every year. The reviewers also reject many papers. But, within a few months of such decline or...

30 August 2019 6,030 5 View

MACS Lambda Local calculation?

Hello everyone, Could someone please provide a working example (or point me to a good resource) of how 'lambda local' (along with lambda BG, 1k , 5k and 10k) is calculated for peak calling in...

24 January 2017 4,509 1 View

What do the Data values in WIG file from MACS mean?

Hello everyone, Could some please explain to me what do the data values in a WIG file mean? I have read the docmentaion about this format on the UCSC webpage and also checked for an answer on the...

16 January 2017 3,839 9 View

How to get generalized equation for dissociation constant of amino acid so I can calculate pH of protein with available pKa values?

17 October 2016 8,114 2 View

preparation of phosphocompost

i want reference on this topic

21 March 2011 7,124 1 View

How to prepare biosurfactant from bacteria?

I want to use biosurfactant to enhance bioremediation of diesel-contaminated soil. So I need to know the biosurfactant extraction procedure from isolated bacteria.

01 January 1970 9,618 3 View

Factors important while culturing of mammalian cells

We have been culturing cells for years now, but we have recently been observing a strange behavior, especially in cell lines like MCF-7, CaCo2, HepG2 and L929, that they are detaching from the...

01 January 1970 2,798 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View