I would like to know the possible methods used to quantify neurotransmitters in biological specimens (Blood/Serum, Tissue) ?

12 November 2022 0 796 Report

I have considered various possibilities including ELISA, and other chromatographic methods. But I am particularly interested in biochemical/colorometric assays which can produce consistent and reliable results.

Badges
Science topic

More Rishikesh M M's questions See All

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

I have calcined my catalysts without flowing air from the compressed air cylinder will there be any problem with my reaction if i use that catalysts?

FYI Catalyst is MFI zeolite Si/Al = 140,25.40.11.5,15 I am using this catalyst to study methanol to DME conversion

25 July 2024 2,486 2 View

How can I measure arsenic concentration in a patient blood?

I intend to get an economical and accurate LAB TEST to measure arsenic concentration in blood sample.

23 July 2024 3,653 2 View

Can it is possible to find the cleaved sequence when a protein cleaved by a heamaglutanin protease (HA/P) by any bioinformatics tools?

Bioinformatics tools like peptide cutter

15 July 2024 6,453 1 View

It is possible to conduct a structured written interview for a qualitative study?

In your opinion, I would like to know if a qualitative study with a structured interview can be conducted in writing. In other words, we sent out open questions and asked the interviewees to...

11 July 2024 4,163 4 View

What is Differential-Fed in microstrip patch ant? How it is design in CST studio? What is process and procedure to do Differential-Fed in CST studio?

What is Differential-Fed in microstrip patch antenna ? How it is design in CST studio? What is simulation process and procedure to do Differential-Fed in CST studio ?

08 July 2024 7,464 1 View

How to know the TM/TE modes in antenna design in CST studio?

How to know the TM/TE modes in antenna design in CST studio? Example: TM01 ,TM02 ,TM10, TM12, TM30 like this... how can identify the antenna is operate the above said mode? can any one explain...

08 July 2024 6,609 2 View

What is co-polarization and cross polarization? How can plot it from CST studio?

what is co-polarization and cross polarization? How can plot it from CST studio? what are steps and process for find out co-polarization and cross polarization from CST studio?

08 July 2024 7,869 1 View

Why in some degradation experiment the results doesn't fit first or second order kinetics ?

In an antibiotic degradation experiment using metals carbon composites to activate PMS, the results doesn't fit pseudo first or second order kinetics however the degradation efficiency is very...

26 June 2024 6,941 3 View

Why carrier Concentration decreases while increasing Temperature ?

Hi All . Recently, I conducted Hall measurement experiments at high temperatures on highly p-doped diamond with a doping concentration of 2.5×10^20 cm−3 During these experiments, I observed two...

23 June 2024 4,763 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,620 1 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View