Which antibiotic produced by Streptomyces sp. has maximum absorption (single peak) at 191 nm in Uv-Vis spectrophotometer?

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Methodological confusions regarding deductive and inductive reasoning, hypothesis and theory test?

Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...

21 February 2021 4,634 6 View

How the standard hydrogen electrode value is convertible into reversible hydrogen electrode?

As much I remember +0.0592*pH, as I am working on CatMAP, so my voltage values are in SHE, not RHE.

18 February 2021 1,685 3 View

Post evaluation of close range photogrammetry experiments ?

I am performing a simple close range photogrammetry test and comparing outcomes of photogrammetry tools such as VisualSFM, Meshroom, Colmap etc. Kindly advice, what post evaluation tests/ criteria...

13 February 2021 6,410 3 View

Train-test split numbers for YoloV3/V4 models?

Dear Research Community, What are the common numbers (in percentages) for splitting the image dataset for training a Yolov3/V4 models? Is there a formal research on this question? Azmyin

08 February 2021 647 3 View

How are the anchor sizes defined in YOLOv3 and YOLOv4?

Dear research community? How are the anchor sizes defined in the cfg files of YOLOv3 and YOLOv4 object detectors? For example from the Yolov4 cfg file...

22 January 2021 4,504 1 View

What are core advantages of 2nd & 3rd generation panel data techniques over the 1st generation techniques?

I am interested to know about the difference between 1st and 2nd and 3rd generation panel data techniques.....

31 December 2020 7,204 7 View

How to construct a combined contour plot of two different text files in python?

As I constructed sperate contour plot for each methane and ethanol but now I wanna draw a combined plot for methane/ethanol ratio (for selectivity purpose)?

30 December 2020 4,531 1 View

Technically is there any difference between policy implications and policy recommendations?

Usually we use the two terms interchangeably in our articles but whether is there any technical difference between them?

26 December 2020 185 18 View

Modeling several ODE in matlab to create a system?

Please I have 4 groups of 8 sets of differential equations that I want to use for simulations. Each set has 8 differential equations to form a group. Each group is coupled with another group...

14 December 2020 8,193 10 View

What are the tools and techniques for making Custom dataset for YOLO?

Dear Research Community. I am new to the field of deep learning based object detection techniques. At this time, I am working on a project which will not use front-facing camera to detect people....

13 December 2020 957 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Negative result on natural product sample cholinergic assay?

i have been doing cholinergic assay on natural product and for some of the samples, i get negative value for the inhibition activity. can anyone help me explain the meaning of the negative value...

17 February 2021 4,118 1 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Can I measure absorbance efficiency with wavelength in a spectrophotometer?

I measure the wavelength of material in a spectrophotometer and it peaks at 275 and its absorbance is 100. I mix it with an adsorbent and it adsorbs the first material, but when I measure the...

16 February 2021 7,515 5 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

What is the actual chemistry going on between iodate and ascorbic acid?

I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...

16 February 2021 8,731 1 View