We used, for our phage ELISA, an anti-M13 antibody from GE-healthcare. This in not sold anymore. Does somebody know a good alternative antibody.
Hi Paula,
Hopefully someone will give you a specific recommendation soon, but if you want to do some looking while you wait, our listings may simplify the process a little: https://www.linscottsdirectory.com/antibodies?q=M13
Just follow "more info" to each supplier's data page. Good luck!
Hi
please , check below link:
http://www.abcam.com/m13-antibody-b62-fe2-hrp-ab50370.html
Russell, Mohammad, Antoni. Thank you very much for your advices. We are testing the one of Syno Biological now, I will let you know how it works. Your listings are in my favorites now.
Paula, after you have tested your antibodies, we encourage you to post a review of how they performed for you, to help other researchers in the future. A good, easy place to do this is pabmabs.com
Good luck with your research.
Hi Paula,
Have you tried the Syno antibodies? Do they work for you? I was planing to buy anti-M13 from a company called Fitzgerald, but I don't know if it is reliable. Their dilution rate looks pretty good though. Product code 61R-M101aBT.
Hi Luiz,
Sorry for the late reply. Yes, untill now the Syno are the best. You can compare them with the GE-healthcare antibodies. They can be coated at 2.5 µg/ml. Store them at -20°C. Once thawed they seem to be more stable at 4°C.
Hi Paula,
Glad to hear you have had some success. As I mentioned before, it would be very helpful for other researchers if you could post a review detailing your experience with these antibodies. We recommend this independent review site: http://pabmabs.com/wordpress/
Hi Paula,
It is fine. Thank you for the answer, I appreciate it.
Hi Russell,
I used your site to find the antibody. I will put comment in the site you recommend.
Paula
Dear Paula:
This is Frank Liu from Taipei, Taiwan. I work for AcadeMab Biomedical Inc., we develop a very good anti-M13 antibody. The binding affinity is much better then GE brand. With your permission, I like to send some free sample for your test and get your test result back to us. Thank you and have a nice day.
Frank@
Hi, we have a new M13 antibody which detects g8p proteins of M13 phage with high sensitivity (10^5 - 10^6 CFU phage) and affinity (1:5000 - 1:75000 dilution is capable for ELISA). And in our in-house testing our new antibody has better performance compared to the antibody from GE. Please refer the detail information in the description page. (https://www.arigobio.com/m13-antibody)