Mohammad Karimi

2 Questions 8 Answers 0 Followers

Questions related from Mohammad Karimi

where is the exact Trypsin sensitive sites of pIII in both M13helper phage and Phagemid vectors? ?

I am planning to make some modifications in my phagemid vector at pIII gene. As trypsyn treatment is the main method for phagemid rescuing, i want to know where is its location on pIII protein to...

06 February 2019 515 0 View

What is difference in use alpha-factor secretion signal in compelete and truncated form for secretion of recombinant protein in pichia pastoris?

For secretion of recombinant protein in pichia pastoris, the most useful secretion signal is alpha-factor secretion signal from S. cerevisiae . In some vectors( e.g. pPICZalpha A, Invitrogen)...

13 October 2017 2,394 3 View