I am analyzing data obtained from a crossover study conducted on same animals evaluating the effect of two different anesthetic drugs on heart rate, respiratory rate, pulse oximetry, rectal temperature and etc over several time points from baseline to induction and every 5 minutes during anesthesia. I would like to detect the effect of anesthetic and time therefore a two way repeated measures ANOVA is required. Do I have to assume sphericity or not and use geisser-greenhouse correction method for this analysis in graphpad prism 8? I would appreciate if anyone with similar experience could reply because the significant results vary considerably.