Which acrylamide:bisacrylamide ratio would be the best for tris-tricine gels?

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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Are protein samples in SDS compatible with ELISA?

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Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

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Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

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Antonina Kokisheva

standard ratio 32:1 (including for small proteins)

http://www.hixonparvo.info/SDS4Peptide.pdf

https://static1.squarespace.com/static/570deb0755598634587afa25/t/57329292859fd0590141c67e/1462932115229/SDS-PAGE+using+Tris-Tricine+system+%28%26+Gradient%29.pdf

Elah Pick

We ended up with a 12% tris tricine gels. Thank you so much Antonina Kokisheva