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Questions related from Elah Pick
We would like to sublone several genes for expression, thus the genomic DNA (sales by ATCC) is not good enough for us (since few of these genes include introns. Any suggestions?
30 May 2023 8,285 0 View
We would like to resolve a globular protein sized 8kD and wonder which acrylamide:bisacryl ratio will be the best (19:1, 29:1, 37.5:1) . We assume that the 19:1 will be the best, but not sure -...
22 April 2019 2,859 2 View
We experienced a fast bleaching of fluorescent staining of S. cerevisiae cells with Nile red. Wondering which anti-fading is suggested for microscopy?
25 October 2017 7,323 8 View
While using TCEP we realized that Di-sulfide bond formation causes proteins to migrate more quickly in SDS-PAGE. Does anyone have an idea how to recognize the specific cysteines involved in this...
01 January 1970 8,795 4 View
