What test should I use?

15 February 2021 911 5 View

How to concentrate a PCR product before sequencing?

Hi everybody, I'm planning to sequence several phytoplasma strains by doing a nested PCR and cloning the resulting amplicons. Standard phytoplasma universal primers pairs will be used (P1/P7...

08 November 2020 2,155 4 View

How remove endotoxins from DEAE-Sepharose?

Hi to everyone! I have to validate endotoxins removal from DEAE-Sepharose I use for the purification of my hormone. The resin undergoes to a sanitization protocol with NaOH. To demonstrate the...

29 October 2020 9,141 4 View

How can I roughly estimate the current denisty in an FPGA?

Hello, I am currently working on the estimation of the electromigration of an FPGA. We just performed the construction analysis so we know all the values of wire width and height for each metal...

22 June 2020 2,743 3 View

What type of proteasome inhibitors is better to use in tissue lysate?

Hi all. I am looking for a proteasoma inhibitor to use in my tissue lysate. I found MG132; lactacystin and PR39. Anyone know which of these is more effective to use in a tissue lysate?, Do I need...

16 April 2020 4,088 3 View

What is the (average) DNA yield (ng) from one single nematode?

Do you know where I can found published information about DNA quantity (in ng) from one single nematode? Any publications/books to suggest me? I Know it seems a simple and basic information, but...

31 March 2020 6,710 1 View

Can I use Arrhenius Relationship to determine Accelerated factor , when test temperature is lower than use temperature?

Hello, I have a question regarding the acceleration factor. We know that the lifetime (TF) of a component can be expressed as: (1) TF=A0exp(Ea/(kT)) where A0 is a constant (component...

01 March 2020 6,635 6 View

Does anyone have experience with fMLP as a neutrophil chemoattractant in a transmigration assay?

I am planning to perform a transmigration assay (transwell plate) in which are involved neutrophils , therefore I will use fMLP as a chemoattractant. I did find some papers that describe "The...

19 February 2020 5,855 3 View

Is there a pigment that can be used to dye PMMA in anisole?

I am looking for an organic dye, soluble in anisole to be able to get coloured PMMA films

30 April 2019 812 1 View

Simultaneous fixation, permeabilization and Phalloidin staining of adherent cells?

Dear all, does anybody have experience with the simultaneous fixation, permeabilization and Phalloidin staining of adherent cells (fibroblast or endothelial cells/cell lines)? Many companies...

25 June 2018 2,381 4 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View