Hi everyone, I have a question with regards to the cell lysis protocol.
I'm considering to do RNA/protein extraction from cornea cells. As a start, we realised that the cornea morphology between keratoconus patients and normal controls are quite different (in terms of corneal thickness, cell structure, etc.).
As the overall corneal structure are more elastic from other tissues due to the presence of fibroblasts (keratocytes) in the stroma layer, where can I find suitable protocols on cornea tissues/cells lysis to refer on? And are there any other suitable methods to lyse these cells for various downstream applications such as gene expression or western blot?
I agree with Sebastian. For RNA, just repeat the procedure he mentioned adding trizol to the mortar.
Appreciate your suggestions. I will look into the recommended methods and proceed accordingly. Thanks again Dr Sebastian Schmitt
and Dr Patrick Jack Spencer.Regards,
Jun Bin
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