Besides Promega, is furimazine, the substrate for Nano Luciferase, available from other sources? I found Promega sells it in kits, which is expensive.
Hello Jiahui Tao.
NanoLuc has been developed by Promega and is a trade mark. So I don´t think that you will find the substrate elsewhere... But maybe I´m wrong.
Greetings
For Furimazine, the fpllowing can be contacted :
Promega India
[email protected], [email protected]
91 1141035028
According to Inouye et al, 2015 (http://dx.doi.org/10.1016/j.pep.2015.02.002), the coelenerazine-h is also a very good substrate for NanoLuc/NanoKAZ.
It can be purchased from many suppliers unlike furimazine.
Although, I didn't see a direct comparison. Furimazine, probably, has lower background.
PS. According to Promega (personal communication), Furimazine gives slightly higher initial bioluminescence and the signal then decays much slower compared to CTZ-h.
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