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I am measuring protein-protein interaction using fluorescence polarization. Is there a tool to calculate theoretic fluorescence polarization vs. M.W. using dyes with different fluorescence...
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Besides Promega, is furimazine, the substrate for Nano Luciferase, available from other sources? I found Promega sells it in kits, which is expensive.
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In Gaussia Luciferase assay, the signal from coelenterazine decays really fast. In NEB's BioLux kit, there is a stabilizer to solve this issue. What is this stabilizer?
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