30 May 2017 1 152 Report

I am in need of an autoclavable dialysis membrane with a MWCO around 100K Da, but I can only find vendors for RC membranes up to a MWCO of 50K Da. Any input on where to find a supplier would be helpful.

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Cleaning up single cell RNA from 10x dataset?

Hello, I am new to single cell RNA data processing and am currently running my data through the initial cleanup steps via Seurat on R. I have previously done this on epithelial organoid data and...

23 December 2020 4,709 1 View

Fluorimeter giving odd results for carbon quantum dots?

I made a Pb sensor using carbon quantum dots (CQDs). I add buffer, CQDs, and a given metal to a quartz cuvette of the recommended type. Before adding a metal, the cuvette will emit a yellow-green...

16 December 2020 9,675 3 View

Recommend resources for developing surveys of technology-users for "needs-analysis" or design criteria?

I am looking for recommendations for good resources or guidelines for developing surveys specifically focused on targeting user's opinions or a needs-analysis in the context of engineering design...

12 December 2020 6,698 6 View

How much salt in a m^3 of water would be needed to prevent evaporation ?

Assume you have 1 cubic meter of water with 1 square meter of surface exposed to the sun. If you kept adding salt to the solution, is there a point at which evaporation would cease? Assuming the...

22 November 2020 5,444 1 View

Automatic cell counting? (Ductal epithelium cells with fluorescence)?

Hello, I am currently conducting a lineage tracing experiment where I need to count GFP+ cells in multiple compartments of ductal epithelium (frozen tissue sections of an inducible Cre mouse with...

20 September 2020 1,855 6 View

Resuspension mutant huntington (mHTT) in conditioned medium?

Hello all. In lab we grow cells to secret mHTT in media that we then collect and subject to centrigufation at 100k G for 2 hours to concentrate the protein and other secreted components of the...

30 August 2020 1,858 1 View

How to improve my CBD to THC conversion method?

Hello Everyone, Let me preface this question by saying that this is all being done under a legal THC/Hemp license in the state of Colorado. I was hoping to get some advice on how to improve my...

27 April 2020 6,292 1 View

Is there literature on pre-steady state "lag" for oxidase enzymes?

Greetings, I'm looking for literature related to a topic I want to write a manuscript on. I'm working with an glycine oxidase enzyme and noticed when I monitor the rate via spectrophotometry and a...

31 March 2020 3,917 2 View

What's going on with my HPLC?

Hi Everyone, I was wondering if you guys had any ideas/suggestions that I can work with to try to fix my HPLC. I have attached a picture of one of our Blanks (100% ACN). This shape is unusual,...

16 March 2020 6,454 5 View

Does anyone have any literature showing 'political or civil engagement' as an important factor for refugees when deciding on repatriation?

This is for research about important issues to repatriation of refugees. Literature (or UNHCR documents) connecting forced migration/repatriation to political/civic engagement would be...

04 March 2020 6,616 3 View

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Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

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02 March 2021 8,481 3 View

Do you know any MR image dataset?

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