In the cytosol or nucleus? Which buffer is suitable to lyse the cell to detect p53 by western blot? Please help me.
And if you mean the TP53 gene, it is located in the short arm of the chromosome 17. (17p13.1)
I am looking for an antimicrobial metal oxide agent for a polymeric implant and I am wondering which one is the best?
21 January 2021 7,068 5 View
Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below...
15 January 2021 6,362 5 View
Hello, I am using ansys fluent to simulate a steady state phenomenon, even though the residual curves are absolutely flatten, pathlines keep changing their patterns over more iteration. I was...
12 November 2020 2,231 3 View
Hey there, I want to simulate & analyze the fracture and fatigue (crack growth) in the rear axle housing of heavy tracks (Volvo company) in Abaqus. My question is which method is suitable for...
12 November 2020 3,377 3 View
It seem individual's attitude to environment are changing rather than last year due to corona virus issues and challenges. What do you think?
27 August 2020 6,394 17 View
The edge detection algorithms are designed to respond to sharp edges which can be caused by noisy pixels. In image analysis edge detection plays an important role and it is one of the traditional...
05 August 2020 2,543 1 View
Dear all, I have used a tie constraint in ABAQUS to attach two surfaces. the meshes of both surfaces are compatible so in front of every node of surface-1 is a node of surface-2. thus the force...
24 May 2020 9,890 3 View
For classification problems, classifier performance is typically defined according to the confusion matrix associated with the classifier. Based on the entries of the matrix, it is possible to...
19 May 2020 3,472 4 View
I am looking for a best dataset with signals of EEG that is used for sleep stage classification.
12 May 2020 5,935 3 View
I am researching QOVI-19 and need a database that contains biosignal data such as electrocardiograms. Is there any dataset?
12 May 2020 9,274 3 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...
23 February 2021 8,609 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...
22 February 2021 6,937 3 View
I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...
22 February 2021 1,777 3 View
Hello, i am a bit curious about my results. I transfected the human cell Line ARPE-19 with a Plasmid containing a GFP tagged p53 (p53-GFP, Plasmid #11770). After 24h i checked under the...
18 February 2021 3,811 7 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...
17 February 2021 1,130 6 View