BSEM/SEM approach?

Hi all! I'm getting into BSEM approach and I am specially concerned about when it is recommended or not to use this model. I have tested a BSEM with a small sample obtaining excellent results,...

07 September 2020 3,919 3 View

Can you help me to find and share material by Ernesto Spinelli?

I am an Italian psychologist, currently I am a professor at the University of Sherbrooke, I work in the field of the existential humanist approach, especially the role of the art in palliative...

23 May 2020 6,863 3 View

Is there an easy way to derivatize silsesquioxanes?

I have a polymer end-capped with a Polysilsesquioxane (POSS) like the one here: https://en.wikipedia.org/wiki/Silsesquioxane#/media/File:Silsesquioxane_T8_Cube.png where *R=i-butyl*. I haven't...

12 March 2020 2,590 3 View

What solvent mixture/ratio should I use for preparing liposomes by REV method?

I have tried with chloroform-methanol (2:1 v/v) but the emulsion produced after sonication is not stable. Also, lipids don't dissolve well in pure ethyl ether.

24 February 2020 2,360 5 View

How can i count the number of instagram posts or tweets associated to a specific location (i.e. a specific street or square)?

I need to find a reliable method for calculating the number of pictures posted on Instagram or of tweets, associated to a specific public open space. The objective is to create a social database,...

16 February 2020 6,175 6 View

Could you suggest any scale to assess parents' satisfaction with their children's schools?

Hi all! I'a looking for a scale to assess parents' satisfaction with their children's schools. I have found some different papers about parents satisfaction but no one seems to offer just what I...

10 December 2018 8,430 3 View

Loading control for WB in High Five insect cells?

30 August 2017 7,901 6 View

Can I extract mRNA from fixed cells?

I want to sort my cells using a marker that require intracellular staining and so, fixation and permeabilation. After that staining, and sorting, can I use these cells as source for mRNA extraction?

01 November 2016 1,286 1 View

No title

30 May 2016 4,100 10 View

What is the best programming language for an open-source computer vision project? Which one would you prefer as potential developer?

We have recently developed a tracking system capable of following several individuals keeping their correct identities in spite of crossings or overlaps (www.idtracker.es). It will be published in...

13 May 2014 9,685 72 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View