I am planning to do EMSA assay with my samples for some known and some new transcription factor. How do I get the oligonucleotide probes for the assay? Somebody please tell me where I will get them or how to design them.
Thank you Alexander sir...
I have an idea to identify the transcription factors binding to a specific DNA region. I don't have any transcription factor candidate, only what I have is a potential promoter region of a gene....
03 April 2013 432 32 View
Is it reliable to use coomassie staining (before transfer) or ponceau staining (after transfer) as a representation of loading control? As most people get problems in selecting a good loading...
03 April 2013 3,618 28 View
It seems to be very difficult to follow a proper pattern of publishing the western blot data. Everybody follows different patterns (ex. in the no. of samples or etc.). Somebody please tell me...
01 February 2013 2,002 5 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?
02 March 2021 4,565 3 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Source to get Impact factor of journals
01 March 2021 6,654 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View