Where can we get SUMO-1 Plasmid?

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Should we normalize sum of two drugs' effect when i compare it to cobination effect in cell lines?

i am analysing data obtained from technical experiment of treated same count of cell lines with two different drugs and their combination. Then i need to find out whether there is a synergy for...

02 March 2019 8,745 4 View

What is the approved method to induce EGFR T790M in mice chemically ?

What is the approved method to induce EGFR T790M in mice chemically ? I am trying to find toxic chemical or drug that has the ability to induce carcinogenicity through double EGFR mutation (L858R...

31 December 2017 478 1 View

Drawbacks of Gene Reporter assay?

If any body using Dual system luciferase Galactosidase gene reporter assay may guide me through the points I should take in consideration to avoid such variability and change in the reproduciblity ?

02 March 2016 1,730 3 View

What is the best method to make IP for HA tagged proteins?

Could you provide me with a protocol for best method of HA pulldown?

11 December 2015 5,883 5 View

What is the order of COD kinetics?

our cell line will be transfected with 2 different plasmids at the same time for 48 hours but the results is not equal between all wells and the results should show us an equality so my question...

11 December 2015 3,968 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View