Hello,
I have started working in the field of optogenetics and I have photonics background. I would like to understand the basis on which an opsin is selected for optogenetic experiments. Moreover, I would also like to learn about choosing the right DNA construct with fluorophores and promoter (including their function etc).
I could only find scattered data online. Can someone help me with any literature or textbooks that explain these concepts with examples for someone who doesn't have biology background?
Thank you.
Best,
Shashank
Hi Shashank,
I hope I'm not too late to offer my help to your question. I have been putting together an Optogenetics Guide to try and answer exactly your question (and others relating to in vivo optogenetics):
https://nicneuro.net/optogenetics-guide/
Please do check it out, and hopefully it will give you a good idea of where to start.
Nic