Does activated charcoal adsorb aflatoxins M1 in milk?

some studies have demonstrated in vitro that activated charcoal can adsorb aflatoxins. more citations would help me have evidence to the scientific...

14 November 2019 211 4 View

I was only able to generate forward sequences for some of my matK, rbcL and ITS1 genes and will they still be useful for my phylogenetic analysis?

I sent some plant DNA for DNA sequencing and apparently, though sequences were sent back, some pairs don't create a consensus sequence. The sequencing company tried to re-sequence, bu to no...

26 April 2019 2,664 4 View

When collecting qualitative data, what systems do people use to keep track of the different data collected?

I would like to know if people have concrete suggestions about or know of bibliography or on line resources that describe how to keep track of the different data collected. For example, if...

02 March 2018 5,892 9 View

Can someone please share a Matlab code to solve a system of (3) non-Linear ODEs (BVP) numerically using Runge Kutta and the shooting method?

30 April 2017 5,566 3 View

Does anyone have experience with growing Expi293F suspension cells in the spinner culture flasks?

I am currently establishing Expi293F cells in my lab using the spinner flasks and not the orbital shaker, but I'm having a few issues: I do not know the best rpm for growth, Cell viability is...

16 January 2017 7,526 1 View

Is there a trick to transfecting BV2 cells with siRNA?

I have tried several different reagents and monitored from 4 to 72 hours, I am not seeing toxicity, but I am not getting siRNA into the cell, no detectable GFP with contro and no knock down with...

16 July 2016 4,525 6 View

I have conducted a Moderation analysis with no interaction effect. Should I change analysis to Multiple Regression?

The moderation analysis was significant for both the IV and Moderating variables, but no significant interaction. This leads me to think I have chosen the wrong analysis and should continue with a...

03 June 2016 1,908 3 View

How to achieve a monolayer of 16hbe14o- cells on a transwell insert?

Hi. I am trying to culture 16hbe14o- cells (human bronchial epithelial cells) on 24/6 well transwell inserts. The cells grow well in T75 flasks but I am struggling to get a mono layer on the...

03 February 2016 6,707 1 View

How to achieve a monolayer of 16hbe14o- cells on a transwell insert?

Hi. I am trying to culture 16hbe14o- cells (human bronchial epithelial cells) on 24/6 well transwell inserts. The cells grow well in T75 flasks but I am struggling to get a mono layer on the...

01 February 2016 2,868 1 View

Can anyone help me solve my issue with thawing embyronic stem cells?

02 May 2015 283 9 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View