No DNA pellet after air drying?

After drying my DNA samples for 20mins under a Bunsen at room temperature I can't see any pellet and no DNA shows up on a gel, despite having pellet in the previous step (precipitation, washing...

08 November 2020 7,775 2 View

What are the social factors that could affect teacher's behavioral intention to use online learning?

What are the social conditions that may influence one's accessibility to digital content?

30 July 2020 3,539 2 View

How to generate a homodimer from a monomer in PyMol or other software?

I performed tertiary structure prediction of my enzyme in Modeller. However, it is predicted to be a homodimer. How can I obtain a homodimer from the monomer obtained by prediction in a reliable way?

07 April 2020 5,264 2 View

Is it necessary to go through an IRB if you want to conduct research independently (no affiliation with a university or organization)?

This question is regarding researchers who are interested in conducting research after grad school but are not affiliated with an organization or university.

23 June 2019 3,779 4 View

Can a PAP pen be used to prevent live cells growing outside a desired border?

I am using a flea cell flow system and need a hydrophobic border but sadly for the slides I am using for imaging they don't have the Teflon border. I was wondering if a PAP pen could be just as...

18 March 2019 202 2 View

How do you modify in-text citations on Mendeley so that they are organised chronologically instead of alphabetically?

Hello, I am using Mendeley as my reference manager. I currently have a merged in-text citation: (Atterbury et al., 2007; Ramesh et al., 1999; Smith & Huggins, 2009) I want it to be...

07 March 2019 393 7 View

Can anyone identify the species of this mold ?

I'm a food science student that likes to try out with different recipes from time to time. So I have made a really odd wine reduction + plain yogurt panna cotta that taste awfully odd (and super...

31 October 2018 563 4 View

Which correction for type 1 error due to multiple correlations?

I'm trying to find a test to correct for type 1 error due to multiple correlations (15 variables). Although Bonferroni is generally considered too conservative, I'm having trouble finding any...

21 February 2016 1,463 10 View

How do I extract DNA from lactobacilli?

21 September 2015 9,866 5 View

Can I boil bacteria to release DNA over extracting the DNA?

I want to use DNA for RAPD PCR, i have only ever used boilates for PCR, but would it be better to extract the DNA using a kit?

07 June 2015 2,559 14 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View