I'm testing a new anti-PSA antibody, and I want to compare it to the one used at the moment in the clinic to detect prostate cancer. Does anyone know how to find out where this antibody comes from? Or who to contact to find out?
Call you clinical lab at your centre they would have the ordering information.
I am out of LN, and I have no choice but to actually just use a dry ice bath to freeze my samples because I need to transport my leaf samples. For context I will be performing RNA extraction of...
14 May 2024 749 1 View
Im working on my dissertation and ive decided to find out what are the most successful factors that go onto a reintroduction project for my chosen species. for example I collected factors such as;...
27 March 2024 9,649 3 View
Hi all! I can't dive into details, but I'm having issues with neutralising sodium citrate pH 5 in a 2-step cDNA synthesis and final RT-PCR. I'm manipulating my reverse transcriptase (100uL...
24 March 2024 7,210 0 View
I need help analyzing enzyme kinetic data. I have data from the Octet K2 system. In my experiment, I load the sensor with our protein of interest (6XHis tag on my recombinant protein to...
20 March 2024 6,373 3 View
Hello everyone ! I am a master student with a specialization in export management and I really need your help regarding the finalization of my research question. I read a couple of articles and...
23 February 2024 7,334 1 View
I have isolated good quality DNA from plant using CTAB method. when i diluted these DNA (20 ng/ul) the DNA get degraded. I have check the diluted DNA on 1% gel but i dont seen the DNA.
13 December 2023 1,807 1 View
Hi, I'm working on designing a camera for a Venus Rover, and I've stumbled upon a UV photodiode that can handle temperatures up to a whopping 550 degrees Celsius. The only catch is, it's built...
13 November 2023 5,699 1 View
I need someone who can at least share with me his/her research about gamification in Primary or in Elementary Education. Thanks
27 September 2023 4,010 1 View
How does attachment style impact relapse prevention in addiction field
15 September 2023 1,013 3 View
Dear all, I'm trying to do the ITS2 secondary structure of a Rhodotorula (basidiomycota). I predicted the ITS2 sequence using ITSx and also ITS2database for comparison. I tried to fold the...
26 March 2023 5,102 0 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.
22 July 2024 9,954 4 View