Brenda Gonzalez

3 Questions 10 Answers 0 Followers

Questions related from Brenda Gonzalez

How is variation in Seahorse XFe24 respirometry controlled?

I have transduce HEK293T cell lines and am attempting to characterize some cellular and mitochondrial phenotypes after KD of my gene of interest. Seahorse is one of my main proposed assays but I...

10 July 2018 7,020 1 View

What are the best transfectable cell lines to use for mitochondrial and senescence studies?

Does anyone know of an easily transfected cell line that is rich in mitochondria (amendable to mitochondrial study) and not immortal? I want to generate cell lines expressing specific genetic...

04 January 2017 1,711 3 View

How do I grow primary B-cells in culture with mitochondrial assay end-points?

Hi RG community, I am fairly new to cell culture to begin with but have been successfully working with primary human fibroblasts, but now I want to do experiments using primary human B-cells and...

06 October 2015 7,731 0 View