Where can I find an anti-mouse Ig purified antibody (not conjugated) with no cross reactivity with human Ig?

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Jonathan E Phipps

have you checked Sigma or ABD Biosciences?

Emilio Carpio

Jonathan

I checked sigma and ABD Bioscience but all I have found are labeled anti-Ig antibodies. I need it unlabeled and purified. Now I've found one from SoutherBiotech, it is a rat anti mouse IgG1, purified and with minimal crossreactivity, Ct no1144-01. I hope it will work for me. any other suggestion will be very welcome

thanks for your help

Ramakrishnan Gopalakrishnan

Available from Jackson immunologicals, Cat#115-001-003 (host goat), 315-001-003 (host rabbit), 515-001-003 (host sheep).

Good luck

Ram

Teresa Gallart

Ram is perfectly right. In my opinion, Jackson is usually the best provider for selection of secondary antibodies of high quality and different characteristics.

Joseph Caruso

Many immunologists are turning to avian immunoglobulins, which are produced from immunizing female chickens or ducks and purifying the maternal Ig from their egg yolk. These often work well in mammalian systems because of their evolutionary distance from mammals. You may want to consider one of these.