I want to transduce cells in vitro with mouse Il6. We have the packaging cell lines, I only need the vector.
I have 2 papers below, but I can't access this, you can help me? Shuai Zhang, Xiaodi Li, Xingyu Zhou, Yuning Wang, Yue Hu, Cloud removal using SAR and optical images via attention mechanism-based...
18 July 2024 9,635 0 View
Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...
15 July 2024 5,153 2 View
Dear all, May I ask a question about indexed by Scopus. 1 month ago, I have published my paper in the Journal name: Challenges in Sustainability in 20/05/2024. However, this Journal has changed...
06 July 2024 6,734 2 View
Some monthes ago on RG I found a nice paper by a bryologist explaining that in a single small tuff of epiphytic mosses different species might co-occur. I remember a plate illustrating type...
01 July 2024 5,102 0 View
I've been fabricating traction force microscopy devices in glass bottom dishes, using a method that first spins a 100 micron PDMS layer, then a ~1 micron PDMS + fluorescent beads mixture layer....
09 June 2024 652 2 View
To be developed
16 May 2024 9,242 0 View
Hi all, I'm trying to validate an ELISA against anti-drug antibodies in which I use a immunized rabbit serum as positive control. Rabbit was a NZR, immunization protocol was "standard" but I...
13 May 2024 2,828 0 View
To be developed.
09 May 2024 5,714 0 View
Hi everybody! I reads some papers or webs on protein sequencing using maldi top MS to sequence digested peptides. But I am wondering that all informations i collected is only about identification...
04 May 2024 3,731 1 View
In the "Edit Load" section in Abaqus, I saw the "Magnitude" value, so how do I get the value of it with Load: Sigma = 1MPa? Does it have any formula to it?
04 May 2024 6,165 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View