Where can I find a cheap display replacement for a Mycycler Thermal Cycler PCR machine?

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Can we use vibratome to section zebrafish larvae for histology?

Hello everyone, I would like to know whether we can use the vibratome to section zebrafish larvae (8-9 days post fertilization) for histology. We have a Leica VT1200S vibratome in our lab....

15 April 2024 1,994 0 View

Zebrafish larvae immunofluorescence protocols?

Hi, May I know the recommended blocking buffers used in zebrafish immunofluorescence staining? In one protocol it advices to use 3% BSA, 1% Triton X-100 and 1% DMSO in PBST (0.1% Tween 20 in PBS)...

21 March 2023 2,390 2 View

Why protein concentrations are very low in J774A1 cells after LPS and ATP treatments?

Hello everyone, I'm trying to extract proteins from J774A1 cells after treating them with 200ng/mL LPS for 4 hours followed by 5mM ATP treatment for another 4 hours to study inflammasome...

29 December 2022 378 0 View

NDVI calculation to predict nitrogen status and disease condition?

NDVI analysis shows about chlorophyll content of the leaves. Lower NDVI values can be occurred due to lack of chlorophyll. Loss of chlorophyll content may be due to nitrogen deficiency in soil and...

11 September 2022 7,211 3 View

How can I get p-type Cadmium selenide by doping ?

I am using spray pyrolsis to deposte CdSe, this compound naturaly n-type and i want to change it to the into the p-type by doping, what is the best material to dope the CdSe to get p-type

08 September 2022 8,036 0 View

Best time point to extract nuclear proteins in RAW 264.7 macrophages?

I would like to know what would be the ideal time to extract nuclear proteins from RAW 264.7 macrophages after they are treated with LPS.

03 May 2022 9,005 1 View

How to find, 5' UTR and 3' UTR regions in a given mRNA strand?

Dear colleagues, When we have a given mRNA sequence, how can I find the exact sequences of its promoter, 3'UTR and 5'UTR regions which lie inside it? Are there any specific analyzing tools? If...

07 July 2021 7,161 2 View

Do plants more preferred to take up amino acids from leaves or roots?

Plants take up amino acids from both roots and leaves. but I want to know which one is prefer?

22 June 2021 9,555 4 View

What is the best way to optimize the crosslinker concentration for protein interaction analysis?

Dear All, What would be the best strategy to optimize a certain crosslinker concentration for my protein interaction studies? Thanks

06 May 2021 1,658 4 View

Why is it difficult to dissolve disodium EDTA to prepare a 0.5M EDTA (pH 8.0) solution?

Dear scientists/ colleagues, I am curious to prepare a 500mL of 50× TAE buffer (Tris Acetate EDTA): using 121g of Tris base(121.14 g mol-1) + 28.55 mL of glacial acetic acid + 50 mL of 0.5 M...

19 March 2021 9,964 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Emilia Paredes Solis Solis

Si no puedes conectar directamente la pantalla, podrías usar un software de control remoto como TeamViewer, AnyDesko Chrome Remote Desktop para controlar el laboratorio desde un ordenador a través de la red. Este tipo de software te permite visualizar y controlar el sistema del laboratorio como si estuvieras frente a él, siempre que ambos dispositivos estén conectados a Internet.

J.M Lahiru Kavinda

Emilia Paredes Solis Solis Muchas gracias. MiCycler es un sistema de PCR antiguo sin conectividad Wi-Fi. ¿Hay alguna otra opción que podamos seguir?

I found a display replacement from AliExpress. Not sure what will be the final outcome. But it was no easy task to find the exact match. I contacted over 50 display sellers and only one seller had that in stock.