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Dear all, I'm considering which assay to use for measuring lipid peroxidation in the neuroblastoma cell line. I came across these 3 options: TBARS, MDA assay, and ELISA. TBARS seems less...
05 February 2022 5,030 4 View
Hi all, I've been reading up on parametric survival analysis, especially on accelerated failure time models, and I am having trouble wrapping my head around the family of distributions. I am...
17 December 2019 345 4 View
Hi everyone, What does it mean when a subscale does not correlate with the total score on the full scale? How does one interpret such a finding? Thank you.
20 May 2019 5,194 3 View
Hi RG, Does anyone know how I might run a parallel mediated moderation analysis on either SPSS PROCESS or MEMORE? I have attached an image of my proposed model, where M1 and M2 are the parallel...
29 January 2019 4,132 2 View
Sanger Sequencing result shows my insert sequence is perfectly right but, as being said in the question, the vector sequence is a mess. Not only is it about a few random substitution or deletion...
02 July 2017 5,252 2 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
How can I calculate IC50 in the DPPH test using graph pad prism software? or suggest a manual method and other software to calculate the IC50 value.
06 August 2024 8,848 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...
24 July 2024 8,696 0 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
How to calculate percentage cumulative drug release when, Total volume of dissolution media = 250ml Added formulation was equivalent to 10mg of drug Sample withdrawn = 1ml each time Sink condition...
15 July 2024 4,192 1 View
Hi, In the 6 well plate, when I am seeding the cells. Do I add 1ml of complete media to one of the wells for the control at this stage or do I wait unit I am treating the plates to do that? Also...
13 July 2024 9,783 1 View
In my country, there is a lot of public outcry in relation to the presence of drug injection and related behaviors in public. Hence at least the bigger cities would probably benefit from the...
08 July 2024 8,903 1 View
Hello everyone reading this topic! It is well known that microcrystalline cellulose 102 is recommended for use in direct compression technology, however, like other grades, it has poor flowability...
08 July 2024 2,807 0 View
