Dear all,
I'm considering which assay to use for measuring lipid peroxidation in the neuroblastoma cell line. I came across these 3 options: TBARS, MDA assay, and ELISA. TBARS seems less specific compared to MDA assay and ELISA, because TBARS detects anything that reacts with thiobarbituric acid, whereas MDA and ELISA reacts only to Malondialdehyde.
However, I found it strange that from the papers I read, it seems that TBARS is commonest, followed by MDA assay, then lastly ELISA? I was wondering if this observation that TBARS is commonest is true, and if so what's the reason behind? Is it just the price? But I don't find TBARS kit being a lot cheaper than MDA assay and ELISA... Is it then the easy of operation, and the consistency of results?
I would also be grateful for any tips while working with lipid peroxidation / oxidative stress assay, assay recommendations and the like.
Thanks in advance for your suggestions.
Chapter Automation of Methods for Determination of Lipid Peroxidation
Although it is automation, the above summarizes Spectrophotometric methods that may be helpful.
Hydroxyl radical attack on unsaturated fatty acids of phospholipid components of membranes produce malondialdehyde (MDA), a lipid peroxidation product. MDA and TBARS levels provide a significant clue to the magnitude of oxidative stress under disease conditions. Better to opt both rather than ELISA.
Dear all, thank you for taking the time to answer the question. Most of you suggested TBARS and MDA over ELISA, hence I also went for TBARS. Will see how it goes soon.
Thanks again!
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