The question is does the addition of the miR mimic cause damage and does the inhibitions prevent damage when PAN is applied to cells
I provide you with the answers to your question below :
The answer to this question depends on the specific experiment that you are conducting. In general, you can damage cells before or after adding miRs to cells, but the results may be different depending on the order.
If you add miRs to cells before damaging them, the miRs may prevent the damage from occurring. This is because miRs can regulate gene expression, and they may be able to suppress the expression of genes that are involved in cell damage.
If you damage cells before adding miRs, the miRs may not be able to prevent the damage from occurring. This is because the damage may have already occurred by the time the miRs are added. However, the miRs may still be able to help cells repair the damage that has been done.
In some cases, it may be necessary to damage cells before and after adding miRs. This is because you may want to see how the miRs affect the cells both before and after they are damaged.
Ultimately, the best way to determine whether to damage cells before or after adding miRs is to conduct an experiment and see what results you get.
Here are some additional resources that you may find helpful:
- Lipofectin-Mediated Delivery of Micrornas: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325450/
- The Effects of Micrornas on Cell Death: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5504923/
- Micrornas and Cell Repair: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5789718/
