Micro CT of fiber reinforced composite - phase identification?

Hi, I have a fiber reinforced polymer matrix composite and I am analyzing it by micro CT but I am unable to reconstruct the fiber phase of the composite. how can I find a solution for this?...

07 January 2021 5,680 1 View

Is calcination necessary for synthesis of Pd deposited CeO2 nanomaterials?

I want to synthesize Pd deposited CeO2 mixed metal oxide composites

03 December 2020 3,195 6 View

How to reduce background of Fluo-4 in flow cytometry of epithelial cells?

I'm trying to measure the calcium increase in epithelial cells (fresh, isolated from mice) after receptor activation using flow cytometry. This is the protocol I follow: after isolating the cells...

29 June 2020 2,231 3 View

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05 June 2018 6,923 11 View

I have analysed the c. igneus leaf extract (methanol) by HPLC with out standard, got 3 major peaks. how to identify the compounds by its peak ?

need clariffication

03 May 2018 6,374 6 View

Can anyone suggest the recommended buffer for incubating enzymatic cell free extract for 48hrs?

I am going to assess the ability of Cell free extract(taken from potential bacterial cells) to degrade the aromatic substance. So for checking the degradation activity i need to incubate the cell...

13 April 2018 2,632 2 View

Reason for Low Reliability of Mini IPIP

03 June 2017 6,353 3 View

Which software is best suitable for Microgrid Simulation?

11 May 2017 9,180 10 View

Is it possible to calculate area measurement for change detected of Multitemporal images

Hi all, Can any one suggest me how to calculate change Area measurement in change detection of multitemporal satellite images.

31 August 2016 4,397 7 View

How can I draw a 1:1 equiline for determining silicate vs carbonate weathering?

23 June 2016 9,076 3 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

How can I calculate teh crystallinity degree of graphene oxide?

I had treat my XRD diffraction pattern by Highscore to calculate the crystallinity degree Xc and I have the formula but I dont no the 2Theta of Ac and Aa to calculate Xc. Is it like polymers...

10 February 2021 6,305 5 View

What is a suitable method to freeze feeders-free, serum free episomal hiPSC?

Hi everyone, I need your professional advice: I started growing episomal iPSC (commercial cell line). I grow them in StemFlex medium. What would you recommend as the most effective freezing...

07 February 2021 8,591 4 View

Does Abaqus plugin AM-Modeler predict the same temperature/crystallinity history as UMATHT user-subroutine?

During AM process, progressively activation of elements can predict the temperature/crystallinity history, including surroundings heat losses and than this data could be used to determine the...

06 February 2021 4,686 1 View

Xrd result of ZnO thin film shown better crystallinity at low temperature and the crystallinity decreases as the temperature increased. why?

I have synthesized ZnO thin films at four different substrate temperature say, 300, 350, 400 and 450C. and the samples were annealed at 450C for half an hour. the xrd result shown better...

04 February 2021 7,641 4 View

Freeze/Thaw of LS174t cells?

Hello World, I have been working with LS174t. Complete Growth Media- 10% FBS/EMEM. ATCC says to freeze with 5% DMSO/CGM. when I defrost my vials, they are not attaching to flask. vials put into...

01 February 2021 8,723 2 View