When there are few options for surfactants (ex., polysorbate 80) what is the best technique to stabilize a nanoemulsion containing fixed oil?

More Helison Carvalho's questions See All

Question about ELISA with the "Cattle IGF 1 (Insulin like growth factor) ELISA Kit" Cat: ELK5630?

Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...

13 June 2024 9,473 0 View

What is the best method for quantifying lipid accumulation in C. elegans images using ImageJ?

We have a demarcation and analysis protocol in ImageJ for images of C. elegans stained with Oil Red O. However, it does not seem to be the best way and we were unable to find an easy-to-execute...

15 April 2024 2,031 1 View

Best way to prescribe periodic boundary conditions (PBC) in Abaqus/Explicit?

Hello, I'm looking to conduct simulations of RVEs (unidirectional discontinuous carbon fibers in resin matrix) utilizing the embedded finite elements technique within Abaqus. I've been successful...

09 April 2024 5,575 1 View

What is the typical % of CD54 and CD86 positive cells in THP-1 cells exposed to DNCB?

DNCB is used as a standard reactivity test for THP-1 cells and supposed to lead to an increase in RFI of at least 200% and 150% respectively. However, I do not seem to find the actual % of...

07 April 2024 3,416 0 View

Problems with conventional pcr. What can it be?

I have a problem with conventional PCR electrophoresis, we are analyzing the deletion of ccr5 delta 32. And this material started to cause problems recently, there was no change in the protocol...

03 April 2024 3,896 3 View

Is it possible to use mean-field homogenisation in Abaqus with Dynamics Implicit?

Greetings, I'm interested in whether it's possible to employ mean-field homogenization (MFH) within Abaqus alongside the Dynamics Implicit (or Explicit) solver. I'm exploring this possibility as...

26 March 2024 3,969 4 View

How to perform a statistical bias correction for wind speed with small in situ time series?

Hi! I wish to perform some kind of bias correction for my historical wind speed time series, which comes from ERA5. However, I just have a few measurements of wind at 100 meters coming from the...

20 March 2024 8,503 0 View

Can anyone share a protocol for quantifying lipids in C. elegans with oil red?

I have already carried out the lipid staining test in C. elegans with oil red, however, in the last tests I am unable to stain all the animals efficiently, most of them do not stain or only have...

22 January 2024 8,696 2 View

Why does the solution containing ivermectin crystallize over time?

11 January 2024 2,140 0 View

How can we improve the control over the quality of suppliers in bigger organizations?

My name is Mirelle and I'm currently taking Business Management classes EAD

27 November 2023 2,591 4 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Does continues partial charging/discharging do any harm to the BESS performance and degradation?

For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...

01 August 2024 9,903 7 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

I am trying to find phonon, and when i run calculations, there is error ?

how to fix this error, from phq_readin : error # 1 DFPT with the Blochl correction is not implemented I am attaching my ph.in file.

23 July 2024 4,649 2 View