Hi,
I have a question regarding the NK cells. I wonder to know when could the NK cells be recovered after Fludarabine and cyclophosphamide treatment in patients?
Thank you so much!
Are you actually asking about the timing of numerical and functional NK cell reconstitution after a Flu/Cy regimen given as lympho-depleting chemotherapy in the setting of a NMA/RIC transplant, lympho-depletion for CAR T-cell therapy...??? Usually, NK cells are the first to recover after restoration of lymphocytes following an hematopoietic transplant...!
Yes, I wonder to know the timing of numerical and functional NK cell reconstitution after a Flu/Cy regimen given as lympho-depletion for CAR T-cell therapy. Do you think NK cells would partly reconstitute after about 21 days of CAR-T infusion? @ Christos Kosmas
Do you think that it would be different in lympho-depletion regimens between U-CAR-T therapy and CAR-T therapy? Then how to make a lympho-depletion regimen? Could it be flexible?
Many thanks!
Xiaomei Yang
NK cells usually recover 15-20 days after an autologous stem cell transplant (ASCT) that is by definition myeloablative, when levels of endogenous IL-15 are very high and these NK cells are premature with an CD62L+/NKG2A+/KIR- phenotype...! I have not found similar data after Flu/Cy lymphodepletion, but I think that NK cell recovery follows similar kinetics as after high-dose chemotherapy and ASCT.
Regarding your question about lympho-depletion regimen, a Flu/Cy protocol usually applied consists of Fludarabine=30mg/m x 3 days and CTX=500mg/m x 3 days...!!!
Christos Kosmas Thank you so much for your response. I really appreciated that!