Freezing BHK cells

17 October 2016 5,034 1 View

Is it possible to reliably predict plant miRNA targets using solely blastn?

Hi everyone! Although I am aware of the existence of several tools for predicting miRNA targets, I would like to know if it is possible to use only blastn to this end in case of plant miRNAs. As...

24 August 2015 8,262 4 View

Is there available in Internet any tissue, organ or cell ontology containing clear connections with biological process terms in Gene Ontology (GO)?

Hi everyone! I annotated thousands of genes of a given organism using GO biological process terms and I would like to map these terms and/or genes to specific tissues/organs. For example, gene A...

29 April 2015 8,439 5 View

Can anyone help with the minimum amount of DNA necessary for Deep sequecing?

What is the minimum amount of DNA necessary for an method (any in deep sequencing) to be able to generate viable sequences?also i would like to know the most sensitive method for this case

07 August 2014 7,729 3 View

How can we develop integrated solutions into the design of buildings?

Discuss and explore new forms of collaboration in the design process of buildings

22 November 2013 3,079 1 View

What questions should i include in my questionnaire?

I am writing my dissertation, about adults who trained at elite status in their youth, i am constructing a questionnaire with the goal of seeing, how many sustained injury, how many sustained...

28 January 2021 4,787 3 View

What are the merits and demerits of GAN and classical data augmentation in plant leaf disease detection and classification systems?

anyone suggest what are the merits and demerits of GAN and classical data augmentation in plant leaf disease detection and classification systems

21 January 2021 5,135 5 View

Do drugs that are injected i.t in rats reach the supraspinal system and how long does it take to clear them from spinal cord?

I am injecting intrathecally (between L5-L6) , in rats, cannabinoid analgesics and I was wondering if they can reach the brain and have effects there as well ? Also I need to pretreat with...

07 January 2021 5,397 4 View

What's the reasonable loading control of nuclear protein in acute injury?

What's the reasonable loading control of nuclear protein in acute injury? Is HDAC1 or Histone H3 a resonable loading control of nuclear protein in acute injury model? How to explain total my total...

06 January 2021 1,607 2 View

Pearsons correlation sample size HELP?

I'm conducting a correlational study about "is there a relationship between depression, acceptance and quality of life among spinal cord injury patient?" But I am having an issue determining the...

06 November 2020 9,798 10 View

Is there some specific drug to induce injury only in muscle fibers without affecting peripheral nerves?

I would like to induce injury in skeletal muscle fibers to study muscle regeneration but I would like to preserve nerve terminals intact. Could it be possible? Is there some specific drug? Thank...

30 October 2020 9,077 4 View

I'm looking for questionnaires to use to determine an athletes mental health after injury. What questionnaires would you recommend best fits??

I'm going into my final year as an undergrad Psychology student and I'm about to embark on my dissertation. I am looking to do my dissertation on sport psychology, looking at the impact of injury...

07 October 2020 8,220 4 View

Is there any harm to eyes for using alcohol based hand sanitizer/ handrub?

With increasing COVID-19 infected cases worldwide, people around the world are practicing strict personal hygiene against infection. Alcohol-based hand sanitizer was one of the best sellers,...

22 September 2020 3,006 12 View

How to increase zebrafish survival rate after spinal cord injury ?

Hi everyone I'm having trouble with the high mortality rate of my zebrafish after surgery. ( the surgery is done based on this article: Becker, C. G., Lieberoth, B. C., Morellini, F., Feldner,...

13 September 2020 6,781 1 View

How do you embed spinal cord in paraffin and how do you recognize ventral horn?

Hi I am going to slice paraffin embedded spinal cord. I have two questions: 1. How do I put the SC in the paraffin block so that it will be in coronal orientation? It is not trivial as the part...

13 August 2020 7,623 2 View