When selecting suitable enzyme for Southern Blot, should it be cut on ORF, LF, RF in wild type and hygromycin, RF, LF in mutant?

Ayat Ibrahiem Esmaeel Al-Laaeiby @Ayat-Al-Laaeiby

27 February 2014 1 626 Report

I am confused about selecting of enzyme for a Southern Blot and where exactly to cut it. I am working with knock out and used hygromycin selectable marker.

Ayat Ibrahiem Esmaeel Al-Laaeiby

Thank you for your answer, do you mean that enzyme should cut in ORF but not on hph?

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