When selecting best primer for ISSR study, consider on PIC value or Shannon index?

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Mostafa Haghpanah

Dear Herath

ISSR is a dominant marker and Estimates heterozygosity can not be possible. So, if Hardy-Weinberg equilibrium calculated, there is an error.

Shone index is not sensitive to the error of dominant markers. there Shone index is used for diversity estimate when we have a dominant markers data.

PIC is an index to detect efficiency of Primers polymorphism. base on PIC you can found out the amount of Polymorphism information.

Finally, Shone index and PIC are two different factors.