I'm wondering which is better for cell seeding, dispersing them into single cells or cell clusters. I thought single cells was better since cells would in this case have a more uniform growth condition (like equal access to nutrients) and each cell would have equally adequate room to proliferate (expecially when the surface gradually gets crowded). Yet as I observed, cells seeded as clusters seem to grow better, being larger and polygonal.
Is it just coincidence and it doesn't really matter, or is there a better option? Thanks in advance for your insight!
Depends on your objective. In most cases, seeding an homogeneous layer of cells is recommended for the reasons you have already stated and in the case of stem cells, and maybe other type of cells, to avoid differentiation, phenotypical changes and/or unequal distribution of a treatment.
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