When performing pharmacological assays, can I prepare dose response curve of specific assay and keep in freezer to be used over few days? Or I should do it fresh every time.
It depends on the stability of the materials. If they are stable enough, you can freeze them. Be sure to seal the materials thoroughly to prevent ice sublimation. When you thaw them out, make sure to mix them completely, since separation of solutes can occur during freezing.
When performing pharmacological assays, it is generally recommended to prepare a dose-response curve fresh for each experiment rather than keeping it in the freezer for multiple days. Here's why:
While it may be convenient to have a dose-response curve readily available in the freezer, it is generally recommended to prepare fresh curves for each experiment to ensure data reliability and accuracy.
When performing pharmacological assays, it is generally recommended to prepare dose-response curves fresh for each experiment rather than keeping them in the freezer and using them over a few days.
The reason for this is that the potency and activity of compounds can change over time, even when stored under appropriate conditions. Freezing the dose-response curves may introduce variability and affect the accuracy and reproducibility of your results. Additionally, certain compounds may be susceptible to degradation or instability upon freezing and thawing, which could further impact the reliability of the assay.
To ensure reliable and consistent results, it is advisable to prepare fresh dose-response curves for each experiment. This involves preparing new solutions of the compounds of interest at the desired concentrations just before conducting the assay. By doing so, you can minimize the potential for degradation, maintain the integrity of the compounds, and increase the accuracy of your pharmacological assays.
It's worth noting that specific assay protocols or experimental conditions may vary, so it's important to consult the literature or protocols provided by the manufacturer for any specific instructions or recommendations regarding the stability and preparation of dose-response curves for your particular assay.
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