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Questions related from Aisha Abdelmalik
I have tried to use this kit to measure released somatostatin from isolated mouse islets, but I had an issue where all measurements were low which should not be the case. doe any body have any...
25 June 2024 1,442 0 View
How to prepare the sections and how to immunostain with three different antibodies? As I am going to use antibody to stain receptor of interest and anti insulin and antiglucagon. How can the three...
29 December 2023 7,135 0 View
Hi , I am looking for a full protocol for immunostaining of mouse pancreatic islets after isolation. I put them in RMPI after isolation. How can I prepare them for staining? and can I do...
06 October 2023 9,314 2 View
I am going to do a radioligand competition binding assay, could any one help me with a protocol. The Kd value of my radioligand is 6.4. And the final volume of the assay is 500microliter, do I...
23 August 2023 2,220 0 View
what is the cause of high scintillation counts in 35s GTP gamma s binding assay, despite of optimizing ligand concentration and all other parameters.
28 June 2023 1,215 0 View
How can preparation of sample for western blotting from isolated pancreatic islet be done?
24 June 2023 7,847 0 View
phd student, I am going to do experiments on islets isolated from mouse pancreatic tissue. to study the effects of some ligands on GSIS. I have some technical questions? how many experiments can I...
16 June 2023 8,179 1 View
When performing pharmacological assays, can I prepare dose response curve of specific assay and keep in freezer to be used over few days? Or I should do it fresh every time.
13 June 2023 2,911 3 View
I am preparing cell membranes for 35s GTP gamma assay, I have two samples of 1.66 mg/ml and 1.4mg/ml protein concentration , and both are 100 microliters volume, how can I dilute them to be kept...
16 May 2023 2,752 3 View
How can I transfect cells with plasmid coding R luc.?
04 April 2023 6,292 3 View
I am doing cAMP assay, using three ligands to study their effect on GPCR. I need to prepare 0.5 mM IBMX in stimulation buffer, and mixing it with cell suspention. Is there any specific method yo...
02 April 2023 4,252 2 View
I am preparing aliquots of doxycycline hyclate, to use with stable cell line. how can doxycycline be filtered?
24 March 2023 2,905 2 View
After inducing cells overnight by doxycycline, how can cells be detached and suspended to perform pharmacological cell based assay? Do they need to be kept serum starved for at least one hour?
18 March 2023 7,525 3 View
hi, I am a PGR student and doing some pharmacology cell based assays. Is there any resources to read from? how % of inhibition in c AMP inhibitory assay?
16 March 2023 2,046 1 View
