To study leaf, stem and root anatomy
To study the change in the profile of endogenous phytohormones
To study the change of activity speed of antioxidant enzymes
There is no best time. Ideally, hormones would start showing observable effects within week.
There are two modes of treatment with phytohormones:
- First, before germination, priming the seeds with desired concentrations of plant growth regulators (PGRs), then allowing germination and assay with different treatments such as biotic and abiotic stresses.
- Second, the best condition is after germination, simultaneously with biotic or abiotic (salinity, cold, drought, heavy metals, etc.) treatments, at the time of 3-leaf of seedling.
Dear Dr. Faride Noroozi shahri
In my opinion, it depends on various factors, including the type of plant, growth conditions (germination, or after germination, growth...) in what conditions it is used. also, You can refer to one of my articles.
https://ecophysiologi.gorgan.iau.ir/article_678616.html?lang=en
The optimal time to assess the response of a plant to exogenously applied phytohormones varies depending on the specific phytohormone and the desired outcome. Generally, it is advised to monitor the plant at intervals, starting from a few hours after application and extending over several days or weeks. This allows you to observe both short-term and long-term effects on growth and other physiological parameters. Keep in mind that different plant species and growth conditions may influence the timing of plant response
It depends on the type of your study and the factors under consideration. Usually, the best time to study for a primary response is 24 to 72 hours after using the phytohormone. However, if you want to examine secondary responses and factors related to biochemical responses, such as antioxidant enzyme activity, it is better to do it by 7 days. Also, if you intend to investigate the effect of phytohormone on plant stress and investigate their effects, it is better to evaluate the target factor in different organs of the plant within 14 to 21 days after the application of phytohormone. Of course, keep in mind that in the third case, you must repeat the phytohormone treatment every 5-7 days.
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