Hey

I work on my thesis, but i have some problem

I know the result is not good, but something is happen, i dont know where/what?

I work on HERC1 520KD, I prepare lysate then did CO-IP with HERC1

Western Blot 8% of Acrylamide Gel, and the transfer is 2.5 hours.

when i add Primary Ab >> anti rector- 192KD- > then Anti Rabbit >> i got the First Picture.

i strip the membrane with Beta-mercapto >> 30 mins at 50C.

to develop another Ab which is HSP 90 the result like this "image 2 " !

Please if you have any Advice, Thank you any way !