Protein size measurement using Zetasizer?

The idea is to measure the hydro- protein sizes in various solvents (eg. with high ionic strength, EtOH/Water, other organic/water solvents), then, due to the extremely low solubility of some...

28 January 2021 297 3 View

How many times should I inject the drug before I take samples for RNA-Sequencing?

I'm going to perform RNA-sequencing of the zebrafish brain with three different treatments, which are naive fish, alcohol withdrawal fish injected with Saline, alcohol withdrawal fish injected...

08 December 2020 4,643 2 View

How do I transform the "Transcripts" into the "Gene symbol"?

I downloaded the expression matrix data "GSE143416_Brain_data_TPM.txt.gz" of GSE143416 from Gene expression omnibus ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi). However, the first column of...

30 November 2020 1,304 3 View

Why can't I get the same natural frequencies, which are calculated by the exported [K] and [M] matrices, compared to the ANSYS Modal analysis result?

First of all, I created a simple solid cubic structure with coarse mesh in ANSYS WB Modal modulus. With the boundary condition is set as 1 face fixed, the first 5 natural frequencies (fn) given by...

23 June 2020 9,836 13 View

How to use Python to optimize molecular structure in batch?

I have a batch of molecular files(.xyz) now and want to optimize geometry of these molecules in batch. Is there some advice about how to process them in batch with Avogadro by using Python? Thank...

23 February 2020 632 4 View

Has anyone observed thermal-induced twin boundary migration?

As we know, due to the tendency to decrease the overall Gibbs free energy, grain boundaries in crystalline materials are subjected to thermal-induced migration, either through a diffusive flux of...

23 December 2019 3,272 2 View

What kind of cells are suitable for cytotoxicity test of nanoparticles?

Hi everyone, I'm currently working on bone-targeting polymeric nanoparticles for treatment of osteoporosis. Just wondering what kind of human osteoblast (or other bone cells) are best suitable for...

03 October 2019 9,077 2 View

Meta-Analysis: How do you compare longitudinal studies?

I'm doing a meta-analysis in R with surgical patients. How do you do a meta-anlayis with longitudinal type studies?? We are looking at radiographic values preop, postop, and follow up (3...

26 July 2019 3,756 2 View

Why I got full length DNA with Taq but partial length with Pfu ?

I want to over lap two DNA sequence A : 378 bps B : 374 bps Taq PCR protocol : A sequence template 1ul (600pg/ul) B sequence template 1ul (600pg/ul) Foward primer 1ul...

30 June 2019 7,127 4 View

Why it is necessary to compare the un-standardized regression coefficients when metric in-variance holds between groups?

Dear Readers: May I ask a silly question regarding metric invariance. Some textbook authors claimed that when metric invariance holds, the comparison across groups of the unstandardized...

01 February 2019 1,671 1 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Efficiency of Proximity Feed Patch Antenna ?

Greetings to all of You! I have searched some research papers for the designing of a proximity coupled patch antenna. Researchers are talking about the impedance bandwidth of the antenna but...

24 February 2021 7,662 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

In Microstrip patch antenna ,How to turn on the diode ,which is placed in the antenna slot with DC blocking capacitor ?

In Microstrip patch antenna ,How to turn on the diode ,which is placed in the antenna slot with DC blocking capacitor ?but entire patch is in AC supply due to antenna feed..

01 February 2021 4,904 3 View

Do head direction cells necessarily encode in an allocentric reference frame?

According to the vertebrate literature, I got the impression that head-direction cells are always anchored to an allocentric reference frame. This can easily be tested with freely moving animals....

27 January 2021 3,821 3 View

Does the pre-synaptic neuron location in a cortical map influences the spatial position of its synapses in the dendrite of the downstream neuron?

Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization? A key element to answer this question is to know if, when...

26 January 2021 1,118 8 View