Mao Yeh

5 Questions 19 Answers 0 Followers

Questions related from Mao Yeh

What is wrong with patch clamp?

Hi, colleges, thank you for your reply. I update my descriptions in my attachment. So sorry for the miswrite of water. It is actually ACSF. I have two questions. Frist, when the pipette into the...

29 July 2014 6,107 20 View

Can anyone help with the difference of hippocampal slices oientation for electrophysiology?

Hippocampal slices for patch clamp and field potential, are prepared from mice using coronal, horizontal, sagital, parasagittal or transverse section. A few papers show that slices orientation...

18 February 2014 7,633 10 View

How to record a stable input-output curve?

I am doing an input-output experiment in the hippocampus. In the control group, the same stimuli elicited variable amplitude of evoked field EPSP. Also, it happens in different slices from the...

26 December 2013 1,348 11 View

How to avoid fluorescence bleaching?

I am doing an experiment about calcium imaging in hippocampal slices. I have two questions. 1. I found that it was hard to load fluo4-am into hippocampal CA1 pyramid cells, so how to improve the...

29 August 2013 7,241 1 View

When I saw "slices evoked spikes with a moderate jitter", I don't know what it means.

And, how to analyze the evoked data to get the parameter "jitter"?

19 August 2013 5,560 3 View