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Questions related from Mao Yeh
Hi, colleges, thank you for your reply. I update my descriptions in my attachment. So sorry for the miswrite of water. It is actually ACSF. I have two questions. Frist, when the pipette into the...
29 July 2014 6,140 20 View
Hippocampal slices for patch clamp and field potential, are prepared from mice using coronal, horizontal, sagital, parasagittal or transverse section. A few papers show that slices orientation...
18 February 2014 7,668 10 View
I am doing an input-output experiment in the hippocampus. In the control group, the same stimuli elicited variable amplitude of evoked field EPSP. Also, it happens in different slices from the...
26 December 2013 1,383 11 View
I do whole cell LTP experiment. When I analyse the data, I find that the baseline is not stable, The access resistance and series resistance is not changed by 20%. Are there some tricks to record...
21 October 2013 308 7 View
I want to retrieve antigen by 1% SDS at 90 ℃. In this situation, can SDS dissolve cell membrane and cause protein loss?
17 October 2013 8,316 7 View
I am doing an experiment about calcium imaging in hippocampal slices. I have two questions. 1. I found that it was hard to load fluo4-am into hippocampal CA1 pyramid cells, so how to improve the...
29 August 2013 7,274 1 View
And, how to analyze the evoked data to get the parameter "jitter"?
19 August 2013 5,590 3 View