Mao Yeh

7 Questions 25 Answers 0 Followers

Questions related from Mao Yeh

What is wrong with patch clamp?

Hi, colleges, thank you for your reply. I update my descriptions in my attachment. So sorry for the miswrite of water. It is actually ACSF. I have two questions. Frist, when the pipette into the...

29 July 2014 6,142 20 View

Can anyone help with the difference of hippocampal slices oientation for electrophysiology?

Hippocampal slices for patch clamp and field potential, are prepared from mice using coronal, horizontal, sagital, parasagittal or transverse section. A few papers show that slices orientation...

18 February 2014 7,671 10 View

How to record a stable input-output curve?

I am doing an input-output experiment in the hippocampus. In the control group, the same stimuli elicited variable amplitude of evoked field EPSP. Also, it happens in different slices from the...

26 December 2013 1,387 11 View

Can someone advise on the whole-cell LTP problem?

I do whole cell LTP experiment. When I analyse the data, I find that the baseline is not stable, The access resistance and series resistance is not changed by 20%. Are there some tricks to record...

21 October 2013 313 7 View

Can someone advise on an antigen retrieve experiment for frozen section?

I want to retrieve antigen by 1% SDS at 90 ℃. In this situation, can SDS dissolve cell membrane and cause protein loss?

17 October 2013 8,319 7 View

How to avoid fluorescence bleaching?

I am doing an experiment about calcium imaging in hippocampal slices. I have two questions. 1. I found that it was hard to load fluo4-am into hippocampal CA1 pyramid cells, so how to improve the...

29 August 2013 7,276 1 View

When I saw "slices evoked spikes with a moderate jitter", I don't know what it means.

And, how to analyze the evoked data to get the parameter "jitter"?

19 August 2013 5,593 3 View