What is the difference between an oligonucleotide primer that used in PCR assay than that used in DNA Sequencing?

Oligonucleotide primers are used to generate bands corresponding to their own gene in PCR assay.

06 September 2020 2,066 7 View

Which company is good for DNA sequencing in Middle East?

I am looking for a good company in DNA sequencing in order to urge my students to cooperate with them and send their research projects to them.

11 October 2019 5,546 1 View

Arabic essay grading dataset?

Can you help me find an arabic essay grading dataset to be used for machine learning?

15 April 2019 3,998 3 View

What are the primer sequences used for APRIL-mRNA expression by RT-PCR?

Can anybody have the correct primer sequences to amplify APRIL gene (TNF-family) for reverse transcription of m-RNA as different articles use different primer sequences as well as the primers for...

26 March 2019 1,494 3 View

How can I judge my time domain measurment accuracy?

Model testing was conducted and we have measurements which varies with time of course such as (speed vs time), (motor current vs time), (force vs time), etc. I just want to measure the accuracy of...

08 January 2019 153 3 View

After submitting a revised manuscript (moderate revision was requested), what are the steps followed ?

15 December 2017 140 90 View

In a set of connected Nodes(routers), how can i measure the distance between them ?

In a set of connected Nodes(routers), how can i measure the distance between them in which i can predict how much time a Packet take to reach next node? Thank You.

06 April 2017 726 8 View

How to register Somatosensory Evoked Potentials (SEPs) using CED Signal software?

I was thinking of recording SEPs in patients with movement disorders. We are using CED signal software in our lab to register MEPs. It has been stated on the software website that it can be used...

28 January 2017 5,897 1 View

How can we estimate some missing values of a spring water flow?

12 August 2016 4,174 10 View

I want to ask about the practical way to transfer N fixing genes between different microorganisms ?

02 February 2016 4,961 2 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

What can we infer from different microbial communities in fish gut from two different locations?

Recently I have been interested in microbial communities from gut & intestine of the same fish species popular in Indonesia marine aquaculture, Grouper (Epinephelus sp.), but are raised in...

18 November 2020 510 5 View

How to calculate power of UV lamp when you know the UV Radiation power density at a distance r ?

13 November 2020 9,820 2 View

How can i package two plasmid system(CRISPR/Cas9) with lamda red recombination in a phagemid to deliver into target cell?

Hi All I am planing an experiment to re-sensitize the colistin resistant gene in Klebsiella pneumoniae. For this i have two plasmid system one carrying the Cas9 and lambda red recombination and...

20 March 2020 1,444 3 View

Can anyone suggest me a protocol for extracting RNA from Gram possitive bacteria cell ?

Dear all, I am attempting to isolate RNA from Streptococcus mutans. Can anyone suggest me a protocol without using commercial kit from companies? Thank you all

31 December 2019 8,888 3 View

How to extract specific type of bacteria from the filters?

If I have HEPA filter containing different types of bacteria in it and that I wish to extract Klebsiella pneumonia out of it, how do I do that?

11 December 2019 3,555 4 View

What is the best media for the culture of Streptococcus pyogenes?

Hello everyone, I want to carry out an antimicrobial Susceptibility Testing using broth microdilution method with 96-well microtiter plate to test the antimicrobial activity of standard...

06 November 2019 4,336 5 View

What is the concentration a streptococcus pyogenes?

What is the concentration in different stages of the disease? What is the concentration in culture under laboratory conditions? Do you know any publications where I will find such information?

09 October 2019 1,275 2 View

Is there a codon optimization tool for gene expression in non-model organisms?

I'm working on a project involving gene transcription and translation rates in Klebsiella pneumoniae and Acinetobacter baumannii. Is there a codon optimization tool for non-model organisms? Most...

09 October 2019 205 2 View

purification of cell wall carbohydrates from Group C Streptococcus (GCS)?

Due to the lack of antibodies to GCS in the market, I would like to prepare my own. I understand that I'll be required to produce purifies cell wall carbohydrate for that purpose. Can someone...

25 September 2019 3,956 1 View