When I am extracting the DNA by CTAB Method form bamboo leaves, the pellet which is obtained at last step when mixed with TE Buffer becomes slimy?

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Houda Kawas

Dear Leelavathy Suresh

Pls. find the attached files

Aljanabi et 1999 An Improved and Ra...d polyphenol free sugarcane DNA.pdf

http://www.scielo.br/pdf/sa/v66n4/a15v66n4.pdf

http://scialert.net/fulltext/?doi=rjmp.2012.65.73

Genomic DNA kusum palas_JNTFP_2010.pdf

http://scialert.net/fulltext/?doi=ajbkr.2011.84.90&org=12

http://nar.oxfordjournals.org/content/23/13/2569.2.full.pdf

http://www.le.ac.uk/bl/phh4/dnaiso.htm

http://en.cnki.com.cn/Article_en/CJFDTOTAL-KMLG200301033.htm

http://en.cnki.com.cn/Article_en/CJFDTOTAL-SWJS200703012.htm

https://plantmethods.biomedcentral.com/articles/10.1186/1746-4811-10-21

http://www.cilr.uq.edu.au/UserImages/File/Plant%20Genomic%20DNA%20Extraction%20by%20CTAB%20_2__Fiona.pdf

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3867630/

http://www.academicjournals.org/article/article1380807891_Ferdous%20et%20al.pdf

http://scialert.net/fulltext/?doi=ijb.2010.293.298&org=11

http://eujournal.org/index.php/esj/article/viewFile/4524/4320

Hoping this will be helpful

Regards

Prof. Houda Kawas

Siji Kavil

I recommend you to include additional chloroform; Isoamylalcohol (24:1) wash just before alcohol wash. If its still not working, its better to use membrane filter from DNA extraction kit in final step to get good quality DNA.

Katharina Pawlowski

It normally helps to grind the plant material together with polyvinylpolypyrrolidone (preferentially with Polyclar AT, but other kinds work as well). Polyclar AT/PVPP binds polysaccharides and is insoluble, so it will go in the cell debris pellet with the polysaccharides and not be a nuisance during the rest of the extraction. Apart from that, I second the recommendation of repeating the extraction with chloroform.