How have professors been able to transition complex ideas and concepts to a virtual environment?

As COVID-19 has forced academia to react instantly to the dynamics of teaching and learning, I am curious to find out how you have been able to transfer complex ideas that you would normally teach...

17 May 2020 8,254 16 View

Can I activate a mixed primary (mouse) glial culture, and then shake microglia off?

Our lab usually uses the shaking method to isolate primary mouse microglia from mixed glial cultures. Till now, I have been collecting microglia, plating them in isolation, and then activating...

17 November 2019 2,048 1 View

What can be an alternative method for determining ECSA of Pt on a carbon paper electrode coated with 10% Pt/C?

I have tried H2 UPD and Cu UPD to no avail. The voltammograms do not show the required peaks on the desorption side to calculate the ECSA. The signal is quite weak to discern over the double layer...

02 August 2018 1,029 4 View

Has anyone had issues with extracting RNA from serum-grown bacterial cells?

In a side-by-side experiment, I got a normal amount of RNA from cells grown in chelexed BHI but very low yields from serum-grown cells. There was more cells in the serum culture, so low cell...

10 May 2018 2,167 3 View

What is the best molecular marker to separate healthy and pathological astrocytes?

I have quantified astrocytes with GFAP in a series of westerns. I would like to know if the lower level of GFAP I am seeing is because it is non-reactive due to a healthy environment or...

06 September 2017 5,783 1 View

My error correction term/speed of adjustment be -1.60529.?

I have performed VECM test with LLB and CPS as my independent variable and GDP as dependent variable. Can i write that In the absence of changes in LLB and CPS deviation of the model from long run...

18 August 2017 684 1 View

Do you have experience or protocols for cryofixation and sectioning of fetal/embryonic pigs?

14 December 2016 2,639 4 View

I have a mixed-design (2: condition X 2: pre-post) but due to a problem with data collection am unable to match pre-test with post-test data. Help?

I am in a bit of a bind with a project evaluating a classroom-based intervention. The design was supposed to be a mixed-design whereby I collected pre-post data from willing students linking...

05 December 2016 938 4 View

Hi, does anyone have an idea on how to purify a molecule with free thiol function without risking its oxidation?

I am interested in getting pure molecules with free thiol but the problem is that during the synthesis I always get a mixture of free thiols and disulphide bridge. After the purification on...

24 May 2016 1,699 1 View

Does anyone have any information regarding a tool to measure attitudes and beliefs of clinical nurses regarding a specific clinical intervention?

Does anyone have any information regarding a tool to measure attitudes and beliefs of clinical nurses regarding a specific clinical intervention?

14 February 2016 5,014 3 View

How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View

How to disaggregate country data into city ones?

I'm using a number of indicators in my research which are not available at a sub-national level. Is there a reliable method for disaggregating the country data into city ones? (preferably with a...

16 February 2021 6,861 3 View

How is Initial GDP measured ?

Dear All, I am working on estimating Debt-Growth Threshold but confused about some points. My questions are; - How do we calculate initial GDP Per Capita? Shall we do it in STATA or excel file?...

15 February 2021 2,677 2 View

How to fix a failed Ramsey's RESET test?

I am making a modified environmental Kuznet's curve for Iceland, the model I have is: dependent variable: ln(CO_2_emissions/capita) independent variables: ln(GDP/capita)...

18 December 2020 9,712 2 View

Panel data with dummy variable as an independent one?

Greetings, so let me explain what I mean in brief. I have n=2 ( two countries), t=30. the dependent variable is GDP growth and the explanatory are 4 continuous ones. the dummy variable ( the...

08 December 2020 8,667 3 View

ARDL bounds test on Eviews: does multicollinearity impede the model from running?

Hi everyone, I hope I can get some help. My group is running an ARDL bounds model to test the Environmental Kuznets Curve in Cyprus. We're trying to perform it on Eviews, but we can't seem to be...

02 December 2020 9,230 4 View

If different variables are stationary at different levels I(0), I(1), and I(2), and are cointegrated, can I run my data by using VECM ?

My data are consisted of 3 exogenous variables (TFIN, SR, and INF), and 1 endogenous variable (GDP). TFIN and INF are stationary at I(0), GDP is stationary at I(1), and SR is stationary at I(2). I...

22 November 2020 8,435 9 View

How do i interpret the long run relationship for VECM in R?

below are my results for the long run output the dependent variable is GDP and the independent variables are CPI, EXCH.RATE and FDI respectively....only the ECT for the dependent variable (GDP) is...

13 November 2020 9,685 1 View

How do I structure my panel data in an event study?

Hey, I am conducting an event study with a short event window (lets say 3 or 5 days). I have a lot of variables, but most of them are fixed over that short period of time (GDP per capita, GDP...

11 November 2020 3,582 6 View

Would you please advise me how to use forecast or predict (out-of-sample)?

Hi, I just run ARDL model in which I have defined "Ln(Budget Deficit/GDP) as Dep-Var and Ln(G/GDP)+Ln(OilRevenue/GDP)+Ln(10%rich/10%poor)+.... some other political-economic indicators as...

19 October 2020 8,641 8 View