I tried human primers without any luck. Bovine primer worked for gapdh but did not work for dll4. I am repeating my experiment to confirm. Sheep primers are not easily available. any thoughts?
There are several domestic & wild sheep genomes. Use those to design good primers.
You can't rush quality.
to get more specific and reliable, primers MUST be designed on the right genome.
try to see the genes you want to work on, get the sequence and find a way for designing.
as a good start for genomes approach, see the UCSC database:
https://genome-euro.ucsc.edu/cgi-bin/hgGateway?redirect=manual&source=genome.ucsc.edu
all the best
fred
Thank you everyone for your answers. I have ordered specific primers and was just curious if anyone has experience with bovine primers.
- Badges
- Science topic