Is there some sort of equation that describes this as other than instantaneous?
So, I want to extract bone marrow from mice for an experiment, but I don't need all tens of millions of cells that I would get. So I was thinking that I could freeze down the left over cells for...
07 May 2024 7,359 1 View
How do I invite them as newcomers to the system they are Sheryl Zimmerman, PhD Sheryl Zimmerman and Phillip Sloane, MD Philip Sloane Also I am part of two separate research teams one is Duke...
02 November 2023 9,684 1 View
Hello! I am looking for stains that can help me visualize the layers of the cerebellum. My goal is to determine quantity of Purkinje cells, granule cells, and Bergmann glia, and integrity of the...
30 August 2023 5,787 3 View
I am working with a nitrogen sensitive species trying to induce somatic embryogenesis. The textbooks I've gone through so far insist that casein hydrolysate - or another reduced nitrogen source -...
09 July 2023 5,736 2 View
Hello, I'm trying to make media for S. cerevisiae that contains everything it needs to survive except any kind of sugar. The goal is to make stock without sugar then add in specific sugars of our...
03 April 2022 6,922 7 View
I am using Gaussian to perform excited-state calculations on a coumarin derivative. In the input file, I have drawn the molecule correctly. The output files always come out with altered bonds...
01 August 2021 6,746 3 View
Is an ontology required to create a knowledge graph or is the schema derived from the relationships in a graph. What I am wondering about is whether the predicate is defined in a knowledge graph,...
20 July 2021 5,622 5 View
I have sent out an online questionnaire as part of my MSc research to a circulation list of some 900 people, whom all have membership of a certain body, and I had imagined of all seniority levels...
25 January 2019 8,932 3 View
We are developing a room temperature vacuum system to measure magneto-electric properties of epitaxial samples. This system will have its temperature accurately controlled using a high performance...
20 August 2018 4,572 2 View
What steps can I take to increase the transformation efficiency of electroporated Cg? When transforming with a plasmid from E. coli, the efficiency is very high and I have ample colonies to choose...
25 July 2018 6,041 1 View
I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...
22 July 2024 412 3 View
Dear researchers. I have extracted data from graphs for meta analysis. I am stuck to find the SD for the obtained data. Anyone who can help me to sort out this problem with formula? Thank...
23 June 2024 2,992 2 View
I have a longitudinal dataset where I am looking to examine whether individuals change their category membership of a variable (with four categories) over four time points (baseline, 1y, 2y, 3y)....
16 June 2024 9,770 10 View
Porn to men is not a big deal. They honestly can’t see how watching a sexy film can be any sort of reflection on their love for their girlfriend. Most don’t understand why she takes offence...
16 June 2024 9,513 0 View
There are two proteins X and Y both containing signal peptides. We want to create a fusion protein of X-Y. X will be displayed on the surface so we retained the signal peptide of X. Further, do we...
11 June 2024 6,117 1 View
Why it is not possible to sort my research items according to number of their citations?
10 June 2024 5,524 0 View
Hello, I got reviver comment " please look into the comparative assessments regarding Ki (inhibition constant); Kd (dissociation constant), and Km "equilibrium constant" of the target...
09 June 2024 10,069 3 View
We produced scFv of an antibody from bacteria. Now we want to establish dissociation Kd of antibody using quantitative elisa. How can i know the expected Kd value (range in nM, or mM)?
04 June 2024 2,602 5 View
While performing gromacs MD simulation after pdbtogmx command using charmm force-field, i have received "Atom P1 in residue NDP 193 was not found in rtp entry NDP with 75 atoms while sorting...
02 June 2024 9,660 8 View
At the risk of asking a stupid question. I'm planning to start isotropic fractionator experiments on insects. The protocol says to make a dissociation solution of 1L of 1% triton x-100 in 40mM...
27 May 2024 821 2 View